A novel 18F-fluorine labeled tracer for imaging mitochondrial membrane potential based on fluorescent probe of JC-1

Research summary

Mitochondria,as "energy factories", are responsible for creating more than 90% of the energy needed by the body to sustain life. Mitochondrial disease is a group of disorders cuased by dysfunctional mitochondria. About 1 in 4,000 children will develope this disease by the age of 10 years.Currently there is no specific method to detect this disorder clinically. This study is designated to develop a novel PET tracer for imaging mitochondrial membrane potential (MMP), which is a key inditor for moniting mitochondrial function.This is an international collaboration project with China: one researcher comes from China and part of project will be performed in China (synthesis of precursor).

Description

The research purposes are as following
1)develop an 18F-labeled molecule as a PET tracer for imaging MMP based on a MMP fluorescent probe, JC-1, as 18F-JC1H.
2)assess MMP using fluorescent probe, TMRM, in cellular level by laser confocal microscope and flow cytometry, and in small animal (rat) by optical imaging.
3)investigate the cellular 18F-JC1H uptake and microPET/CT imaging of this tracer.
4)develop a mathematical model to describe the kinetics of 18F-JC1H in vivo.
5)correlate the cell uptake of 18F-JC1H with quantified MMP.
6)image rat/mouse models of mitochondrial diseases (collaboration with other research groups) using 18F-JC1H.

We hope that this novel PET tracer (18F-JC1H) can be used to study and diagnose the mitochondrial disease.

More information

Research publication

Research info

Research title
A novel 18F-fluorine labeled tracer for imaging mitochondrial membrane potential based on fluorescent probe of JC-1

Research timeline
1.10.2014 - 1.8.2017

Keywords
health Medicine Mitochondria Nuclear medicine

Region
Asia

Countries
China, Finland

Institution
Turku PET Centre
Turku

Funding instrument
Other

Project budget
0 - 200,000 euros

Head of research
Chunlei Han

Research team
Xiaoqing Zhuang, Olof Solin;Merja Haapanranta-Solin, Tove Grönroos;Juhani Knuuti

Partners
Deparment of Nuclear Medicine of Niningxia Medical University Hospital, Yinchuan, China

Contact information
Chunlei Han
(office)+358(0)23130493/(mobile)+358(0)503419568
chunlei.han@tyks.fi

Record last updated
28.8.2015